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Showing 38 results

The efficacy of DNA sequencing on samples of terrestrial invertebrates 2018/2019.

Published online: 09 Aug 2022

Published by: Natural England

Authors: Rees, H. C. & Maddison, B. M. & Owen, J. P. & Baker, C. A. & Bishop, K. & Gough, K. C. & Webb, J. R.

Content type: Reports

This publication reports on the accuracy of DNA species identification for invertebrates, and investigates also on the reliability of the BOLD databas...

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An assessment of the fungal conservation value of Hardcastle Crags (Hebden Bridge, West Yorkshire) using NextGen DNA sequencing of soil samples.

Published online: 29 Oct 2021

Published by: Natural England

Authors: Griffiths, G. W. & Cavalli, O. & Detheridge, A. P.

Content type: Reports

Soil samples were collected from 14 grassland areas (each ca. 900 m2) in the Hardcastle Crags area, near Hebden Bridge, West Yorkshire (Hollin Hall, C...

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Assessing metabarcoding methods for re-analysis of pond DNA samples: Towards a new metric for assessment of pond condition.

Published online: 24 Jan 2024

Published by: Natural England

Authors: Ward, G. & Robertson, C. & Ryder, D. & Bass, D.

Content type: Reports

The aims of this study were to determine whether the quality and quantity of plant and eukaryote DNA present in the district level licensing (DLL) sam...

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Chinese mystery snail, Cipangopaludina chinensis, phylogenetic analysis and barcoding: Pevensey Levels, Sussex; and Southampton, Hampshire.

Published online: 08 Oct 2024

Published by: Natural England

Authors: Rees, H. C. & Measures, G. H.

Content type: Reports

This study was commissioned to build on the qPCR assay developed for detecting Cipangopaludina chinensis by ADAS in 2021 (NECR410). The qPCR successfu...

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UK Barcode of Life: 2023 project - DEFRA Centre of Excellence for DNA Methods.

Published online: 05 Sep 2023

Published by: Natural England

Authors: Price, B. & Cristovao, J. & Sivell, O. & Hall, A. & Hall, D. W. & White, O. & Wilbraham, J. & Brodie, J. & Blagoderov, V. & Whiffin, A. & Cuber, P. & Davis, R. & Misra, R. & Leatherland, D. & Clark, K.

Content type: Reports

DNA-based methods offer a significant opportunity to monitor individual species and species assemblages where appropriate, for example those that may ...

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GenePools: Investigating the life that dwells in garden ponds.

Published online: 22 Jun 2023

Published by: Natural England

Authors: Rees, H. C. & Baker, C. & Kane, S. & Owen, J.

Content type: Reports

This study describes the use of GenePools to determine the combination of citizen science with eDNA metabarcoding techniques to measure biodiversity w...

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UK Barcode of Life: 2022 project update.

Published online: 06 Jan 2023

Published by: Natural England

Authors: Price, B. & Cristovao, J. & Hall, A. & Cuber, P. & Geeves, C. & Salatino, S. & White, O. & Davis, R. & Scott, B. & Misra, R. & Leatherland, D. & Clark, K.

Content type: Reports

Gaps in DNA reference libraries of UK species were identified by the Defra DNA Centre of Excellence Working Group as one of the main barriers preventi...

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Testing the validity of using eDNA for carbon origin analysis from sediment cores.

Published online: 28 Nov 2024

Published by: Natural England

Authors: Pagaling, E. & Buswell, V. G. & Cooper, A. & Hedley, P. & Cock, P. J. A. & Robinson, L. & Morris, J.

Content type: Reports

The present pilot study tested the validity of metabarcoding to characterise blue carbon stocks in the UK using sediments from the Solway Firth. This ...

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Pilot study to validate an environmental DNA sampler for monitoring inshore fish communities.

Published online: 24 Mar 2021

Published by: Natural England

Authors: Mynott, S.

Content type: Reports

The utility of environmental DNA (eDNA) techniques for monitoring species diversity are gaining acceptance amongst industry and government decision-ma...

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An examination of the accuracy of a sequential PCR and sequencing test used to detect the incursion of an invasive species: the case of the red fox in Tasmania.

Published online: 10 Jun 2015

Authors: Ramsey, D. S. L. & MacDonald, A. J. & Quasim, S. & Barclay, C. & Sarre, S. D.

Content type: Journal article

Journal title: Journal of Applied Ecology

Polymerase chain reaction (PCR) diagnostic tests are increasingly applied to the identification of wildlife. Yet rigorous verification is rare and the...

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